Description
The method employs sandwhich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Mouse Interleukin 8, IL-8 present in the sample are bound by the antibodies. Then, a HRP (horseradish peroxidase) conjugated secondary antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse Interleukin 8, IL-8 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm